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MitoScene? Green I
产品概述:
产品参数
外观:可溶于 DMSO、DMF 的红色固体
Ex/Em: 490/523 nm (in MeOH)
贮存条件:-20℃避光保存
保质期:有效期见外包装
产品先容
MitoScene TM Green I 是一种线粒体绿色荧光染料,可在纳摩尔水平对线粒体进行染色标记,该染料具有膜透性,且在线粒体膜上积累而呈现明亮的荧光。该染料在线粒体中的定位不依赖于线粒体膜电位,该染料可以染活细胞或甲醛固定细胞,但是在细胞固定、透化之后,会导致荧光信号减弱或丢失。
注意事项
1. MitoScene TM Green I 使用前,先进行梯度稀释,再加入含有细胞的培养基中,以防止局部染料浓度过高,影响染色效果。
2. 荧光染料均存在淬灭问题,请尽量注意避光,以减缓荧光淬灭。
3. 为了您的安全和健康,请穿实验服并戴一次性手套操作。
引用及参考文献:
1.Circadian clock gene NPAS2 promotes reprogramming of glucose metabolism in hepatocellular carcinoma cells
PengYuana,TaoYang,JiaoMu,JingZhao,YiYang,ZhaoyongYan,YiranHou,ChengChen,JinliangXing,HongxinZhang,JibinLi
Cancer Letters Volume 469, 28 January 2020, Pages 498-509
引用文献
1.Staining of mitochondrial membranes with 10-nonyl acridine orange, MitoFluor Green, and MitoTracker Green is affected by mitochondrial membrane potential altering drugs.
应用方向:分析HL60细胞线粒体膜电位
2.Efficacy of MitoTracker Green and CMXrosamine to measure changes in mitochondrial membrane potentials in living cells and tissues.
应用方向:对线粒体特异染色,线粒体形态和功能的研究
3.MitoTracker Green labeling of mitochondrial proteins and their subsequent analysis by capillary electrophoresis with laser-induced fluorescence detection.
应用方向:标记线粒体是通过与线粒体的蛋白特异结合
4.Mitotracker green is a P-glycoprotein substrate.
应用方向:染料是一种P-糖蛋白底物
5.Alveolar macrophages from subjects with chronic obstructive pulmonary disease are deficient in their ability to phagocytose apoptotic airway epithelial cells.
应用方向:追踪16HBE气道上皮细胞的有丝分裂
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