澳门游戏平台-正规手机网投平台

中文 ENGLISH

 “完美新发明”GelBlue——安全凝胶电泳新时代 

  • 2× SYBR Green qPCR Master Mix

    产品货号: S2014

    产品规格: 20μL×100T/20μL×500T

    目录价(元):500/1200

    可替代市场同类产品

    大包装询价


产品概述:

产品内容

组分

100T

500T

      A. 2×SYBR Green Master Mix   

1 mL

   5×1 mL   

B. 10 × ROX reference dye

   0.5 mL   

1 mL

组分A包含SYBR Green dye, dNTP, PCR buffer(含Tris和MgCl2),热启动Taq聚合酶;组分B是10 ×ROX 参比染料。

储存条件
长期保存请在-20℃避光保存,有效期见外包装。使用前,将产品室温下解冻,并轻轻涡旋混匀,解冻后所有实验应在冰上操作。该产品可以重新冷冻储存。

产品先容
SYBR Green 是一种结合于所有dsDNA双螺旋小沟区域的具有绿色激发波长的染料。SYBR Green 与dsDNA 结合荧光信号会增强800-1000倍,是一种常用的qPCR荧光染料。具有高灵敏度,信噪比高等优势,可应用于基因表达差异分析,基因芯片等。

注意事项
1.参照染料ROX:对于某些固定型号的仪器,必须添加ROX 才能精确测定Ct值。ROX的使用浓度可以参照下表。ROX会给熔解曲线分析造成一定的背景干扰,因此,为了避免 ROX杂峰干扰,在应用App的“Passive Reference Dye”中 不要选择检测ROX荧光值选项,然后进行数据的收集、分析。
2. 退火温度:退火温度应根据引物Tm值设定,通常是50 -60℃为佳。不过,引物Tm值(和扩增温度)应该设计尽可能地接近60℃(但仍在50 - 60℃范围内),减少退火和变性温度之间的差距。这样温度增加所需时间更少,进而扩增效率更高。


说明书:


   UE-S2014

常见问题解答:


◆Ct值出现过晚
1.扩增效率低,反应条件不够优化:重新设计引物;更换灵敏度更高的试剂;改用三步法反应,降低退火温度
2.反应成分降解或模板量偏低 设置阳性对照;检查RNA质量;提高反转录效率
3.PCR产物太长:重新设计引物,扩增长度建议100-200bp

◆无Ct值或无信号
1.循环数偏少:建议扩增循环数35以上,可以根据具体情况增加循环数至45
2.模板不足或降解:检测RNA质量;提高反转录效率;避免cDNA反复冻融
3.引物失效 :设置阳性对照;凝胶电泳检测是否存在扩增产物

◆扩增曲线S型异常
1.ROX添加不当:根据机型选择合适的ROX
2.模板浓度过高:适当稀释模板,减少扩增抑制
3.基线设置不正确:基线起始于3-10个循环,终止于指数扩增前

◆溶解曲线不是单峰
1.引物设计及用量:重新设计退火温度较高(58-62℃),无错配,Blast对比特异的引物;引物用量合适(0.2-0.4μM)
2.模板有基因组污染:优化RNA提取方法;选择合适的反转录试剂;跨内含子设计引物
3.基因表达量低:增加反转录效率,适当提高模板量
4.扩增体系不佳:使用灵敏度高,特异性强的试剂


◆标准曲线线性关系不佳
1.加样误差:引物预混后再分复孔:矫正移液器,使用硅化枪头;使用文库稀释液稀释标准品
2.标准品降解:避免反复冻融:电泳检测发现降解后重新制备稀释标准品
3.模板浓度高或有抑制物:改变稀释精度,增加稀释梯度
4.引物或探针不佳:重新设计引物和探针

◆NTC有扩增
1.水,试剂或引物有污染:分装水和引物,试剂优先加入到体系中
2.引物二聚体:优化引物设计,提高退火温度(58-62℃);降低引物浓度(0.2-0.4?M)

◆扩增效率低
1.反应条件不够优化:降低退火温度,使用三步法
2.PCR抑制物:优化核酸纯化方法;适当稀释模板降低抑制物影响
3.引物设计:引物避免错配;扩增片段长度在100-200bp


引用及参考文献:


1.Genome-wide identifi cation and transcriptome-based expression analysis of sox gene family in the Japanese fl ounder Paralichthys olivaceus
YU Haiyang, DU Xinxin, LI Xiaojing, QU Jiangbo,ZHU He, ZHANG Quanqi, WANG Xubo
Journal of Oceanology and Limnology (2018)  


2. 
Whole-genome resequencing from bulked-segregant analysis reveals geneset based association analyses for the Vibrio anguillarum resistance of turbot(Scophthalmus maximus)
Zhang KHan MLiu YLin XLiu XZhu HHe YZhang QLiu J.
Fish and Shellfish Immunology(2019)


3.
Edwardsiella tarda-induced miR-7a functions as a suppressor in PI3K/AKT/GSK3β signaling pathway by targeting insulin receptor substrate-2 (IRS2a and IRS2b) in Paralichthys olivaceus
YuezhongLiuYuxiangLiuMiaoHanXinxinDuXiumeiLiuQuanqiZhangJinxiangLiu
Fish and Shellfish Immunology(2019)


4.
Duck RIG-I restricts duck enteritis virus infection
Hong Huo,Yue Wang,Dongfang Wang,Yiping Wang,Xiaohan Chen,Lili Zhao,Hongyan Chen.
Veterinary Microbiology(2019)

5.Comparative studies on duplicated tdrd7 paralogs in teleosts: Molecular evolution caused neo-functionalization
Bo Wang,Xinxin Du,Huizhen Wang,Chaofan Jin,Chen Gao,Jinxiang Liu,Quanqi Zhang
Comparative Biochemistry and Physiology Part D, genomics & proteomics(2019)

6.The Evolution and Possible Role of Two Sox8 Genes during Sex Differentiation in Japanese Flounder (Paralichthys olivaceus)
Haiyang Yu ,Yujue Wang ,Xiaojing Li,Feifei Ni,Minmin Sun,Quanqi Zhang,Haiyang Yu,Xubo Wang
Molecular Reproduction and Development(2019)

7.Inhibition of arsenite methylation induces synergistic genotoxicity of arsenite and benzo(a)pyrenediol epoxide in SCC-7 cells
Youxian Li,Man He,Beibei Chen,Bin Hu
Metallomics,11(1) · October 2018

8.Genome-wide identifi cation and transcriptome-based expression analysis of sox gene family in the Japanese fl ounder Paralichthys olivaceus
YU Haiyang;DU Xinxin;LI Xiaojing;QU Jiangbo;ZHU He;ZHANG Quanqi;WANG Xubo
Journal of Oceanology and Limnology(2018-05)

9.Evolutionary significance and regulated expression of Tdrd family genes in gynogenetic Japanese flounder (Paralichthys olivaceus)
BoWanga,HuizhenWanga,HaofeiSong,ChaofanJin,MeitingPeng,ChenGao,FanYang,XinxinDu,JieQi,QuanqiZhang,JieCheng
Comparative Biochemistry and Physiology Part D, genomics & proteomics

10.Expansion and Evolutionary Patterns of Glycosyltransferase Family 8 in Gramineae Crop Genomes and Their Expression under Salt and Cold Stresses in Oryza sativa ssp. Japonica
Weilong Kong, Ziyun Gong, Hua Zhong, Yue Zhang, Gangqing Zhao, Mayank Gautam, Xiaoxiao Deng, Chang Liu, Chenhao Zhang and Yangsheng Li
Biomolecules 2019, 9(5), 188

11.Sugar Transporter Proteins (STPs) in Gramineae Crops: Comparative Analysis, Phylogeny, Evolution, and Expression Profiling
Weilong Kong, Baoguang An, Yue Zhang, Jing Yang, Shuangmiao Li, Tong Sun and Yangsheng Li 
Cells 20198(6), 560

12.Rapid evolution of piRNA pathway and its transposon targets in Japanese flounder(Paralichthys olivaceus)
HaofeiSong,ChangjinXing,WeiLu,ZeyuLiu,XuboWang,JieCheng,QuanqiZhang
Comparative Biochemistry and Physiology Part D: Genomics and Proteomics(Volume 31, September 2019)

13.LGP2 plays a critical role in MDA5-mediated antiviral activity against duck enteritis virus
HongHuo,LiLiZhao,DongFangWang,XiaoHanChen,HongYanChen
Molecular Immunology Volume 116, December 2019, Pages 160-166

14.Glypican-3 Enhances Reprogramming of Glucose Metabolism in Liver Cancer Cells
Gebing Yao, Jikai Yin, Qing Wang, Rui Dong,  Jianguo Lu
BioMed Research International Volume 2019, Article ID 2560650, 11 pages

15.SIK2 enhances synthesis of fatty acid and cholesterol in ovarian cancer cells and tumor growth through PI3K/Akt signaling pathway.
Jing Zhao, Xiaohong Zhang, Tian Gao, Shanci Wang, Yiran Hou, Peng Yuan, Yi Yang, Tao Yang, Jinliang Xing, Jibin Li,Shujuan Liu 
Cell death &disease(11, Article number: 25 (2020))

16.miR-430a regulates the development of left-right asymmetry by targeting sqt in the teleost
Fan Yang,Jie Qi
GENE Volume 745, 30 June 2020, 144628

17.陆地棉转录因子 GhWRKY72 基因克隆及表达分析
 封润霞,温小杰,王昭玉,张雪,张书玲,刘建凤
河北农业大学学报 2019,Vol.42 Issue(6):10-15

18.Antibacterial functions of a novel fish-egg lectin from spotted knifejaw (Oplegnathus punctatus) during host defense immune responses
Kai Zhang,Xiaobing Liu,Xuemei Li,Yuxiang Liu,Haiyang Yu,Jinxiang Liu,Quanqi Zhang
Developmental and Comparative Immunology (Volume 111, October 2020, 103758)

19.Micro-Evolution Analysis Reveals Diverged Patterns of Polyol Transporters in Seven Gramineae Crops
Weilong Kong,Tong Sun,Chenhao Zhang,Yalin Qiang,Yangsheng Li
Frontiers in Genetics

20.UEIris II RT-PCR System for First-Strand cDNA Synthesis Kit; Minerva Super Fusion Cloning Kit; 2 x SYBR Green Mixture
Qiang Fang,Yueqing Li,Baofeng Liu,Xiangyu Meng,Zhongzhou Yang,Song Yang,Tingting Bao,Shadrack Kimani,Xiang Gao,Li Wang
Plant Physiology and Biochemistry(Volume 154, September 2020, Pages 439-450)

21.The functional differentiation of four smad4 paralogs in TGF-β signaling pathway of Japanese flounder (Paralichthys olivaceus)

Haiyang Yu,Yujue Wang,Chaofan Jin,Yuxiang Liu,Yan He,Quanqi Zhang
Cellular Signalling(Volume 71, July 2020, 109601)

22.Growth differentiation factor 9 (gdf9) and bone morphogenetic protein 15 (bmp15) are potential intraovarian regulators of steroidogenesis in Japanese flounder (Paralichthys olivaceus)
Haiyang Yu,Yujue Wang,Mengya Wang,Yuxiang Liu,Jie Cheng,Quanqi Zhang
General and Comparative Endocrinology(Volume 297, 1 October 2020, 113547)

23.Growth differentiation factor 9 (gdf9) and bone morphogenetic protein 15 (bmp15) are potential intraovarian regulators of steroidogenesis in Japanese flounder (Paralichthys olivaceus)
HaiyangYu,YujueWang,MengyaWang,YuxiangLiu,JieCheng,QuanqiZhang
General and Comparative Endocrinology Volume 297, 1 October 2020, 113547

24.Quantitative proteomic analysis of castor (Ricinus communis L.) seeds during early imbibition provided novel insights into cold stress response
Xiaoyu Wang,Min Li,Xuming Liu,Lixue Zhang,Qiong Duan,Jixing Zhang
Int. J. Mol. Sci. 2019, 20(2), 355

25.Normal vitreous promotes angiogenesi via the epidermal growth factor receptor
Mengling You,Xiaobo Xia,Haibo Li,Jiayu Wu,Rong Rong,Zhou Zeng,Kun Xiong,Jufang Huang,Luosheng Tang,Hetian Lei,Wenyi Wu,Dan Ji
FASEB Journal

返回顶部

更多>> 技术支撑

  • 地址:苏州市相城经济开发区观塘路1号 C座10F
  • 电话:1号技术18551299082、2号技术18551296769、3号技术18551290363
  • 电话:1号销售18551296519、2号销售13182673060、3号销售(工业)18551296656

在线
客服

在线客服服务时间:9:00-24:00

  • 1号技术支撑
    3186249013
  • 2号技术支撑
    3111274163
  • 3号技术支撑
    3106481410
  • 1号销售(渠道)
    3448192208
  • 2号销售(渠道)
    3613306134
  • 3号销售(工业)
    2738609142

客服
热线

客服热线
0512-69571710、18551296680

1号技术热线 185512990822号技术热线 185512967693号技术热线 185512903631号销售(渠道) 185512965192号销售(渠道) 131826730603号销售(工业) 18551296656

关注
微信

微信服务号
顶部

澳门游戏平台|正规手机网投平台

XML 地图 | Sitemap 地图